Contact Information

Reeta Huhtala
tissue-service@helsinki.fi
+358-2941 25260
Room A129a

Helena Taskinen
tissue-service@helsinki.fi
+358-2941 25270
Room B117b

Street address:
Haartmaninkatu 8
(Biomedicum Helsinki)
00290 Helsinki

Mailing address:
P.O. Box 63
00014 University of Helsinki
Finland

Staining Services

  • HE-staining
    Responsible person: Helena Taskinen
  • Immunohistochemistry and insitu hybridization
    Responsible person: Reeta Huhtala

The immunohistochemical stains and in-situ hybridization are performed by the automated system InsituPro VSi (Intavis, Germany). The system can process up to 60 slides or 32-60 whole-mount samples per run. One run takes about 24-52 h depending on the protocol. The user only needs to provide samples and antibodies or probes.

Using the system makes the staining process highly repeatable and convenient for large numbers of samples. A test run to determine the appropriate dilution of new probes or antibodies is recommended before a full-scale experiment.

The hematoxylin-eosin staining is made in the automated system Tissue-Tek DRS that can process multiple slides in one go.

Automated in-situ hybridization (ISH) on zebrafish brain sections on slides combined with manual immunohistochemical (IHC) staining. Red: c-Fos ISH with FastRed substrate, green: dopaminergic marker, manual IHC. S. Semenova, 2012

Automated in-situ hybridization on mouse brain using a syndecan 3 probe. X. Zhao 2012
A 3 dpf zebrafish larva, automated whole mount in-situ hybridization with a tyrosine hydroxylase 1 probe. R. Korhonen, 2012